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článek v časopise v databázi Web of Science
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Cells generate mechanical forces to maintain normal cellular function or play a role in developing
pathological processes. The mechanical force called the traction force can be estimated from the pillar
deflection using a polymeric micropillar array. Here, we develop a transparent membrane of hollow coneshape high aspect ratio (AR) parylene-C micropillars by the molding method. The membrane of the pillar
array is exposed after the etching of the silicon mold and the residual silicon serves as the cultivation
chamber. The AR and spring constant (k) of micropillars are estimated to AR ≈ 10 and k ≈ 0.349 N·m−1.
The spring constant of developing micropillars is 3.5-times decreased compared to cylindrical, non-hollow
pillars. This slightly tune the elastic properties of micropillars. The array is further shown as the
mechanosensor detecting the change of cellular tension during hyperosmotic stress. The traction force of
cancerous PC-3 cells is estimated from pillar deflections by image analysis. Additionally, the cell volume
and surface area are measured using a digital holographic microscope (DHM). The results show that the
molding technique can be used to develop high AR parylene-C micropillars and that this array can serve as
the mechanosensor of cellular processes.
pathological processes. The mechanical force called the traction force can be estimated from the pillar
deflection using a polymeric micropillar array. Here, we develop a transparent membrane of hollow coneshape high aspect ratio (AR) parylene-C micropillars by the molding method. The membrane of the pillar
array is exposed after the etching of the silicon mold and the residual silicon serves as the cultivation
chamber. The AR and spring constant (k) of micropillars are estimated to AR ≈ 10 and k ≈ 0.349 N·m−1.
The spring constant of developing micropillars is 3.5-times decreased compared to cylindrical, non-hollow
pillars. This slightly tune the elastic properties of micropillars. The array is further shown as the
mechanosensor detecting the change of cellular tension during hyperosmotic stress. The traction force of
cancerous PC-3 cells is estimated from pillar deflections by image analysis. Additionally, the cell volume
and surface area are measured using a digital holographic microscope (DHM). The results show that the
molding technique can be used to develop high AR parylene-C micropillars and that this array can serve as
the mechanosensor of cellular processes.
