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The ordered nanopores surfaces has been studied in the field of biosensors for
the detection of affinity interactions such as antigen-antibody or nucleic acid hybridization.
Nanopores are frequently fabricated by anodic oxidation of aluminium. This method is
practically simple method of highly ordered pore formation, but is not suitable for massive
production. The principle of detection is usually based on the degree of pore blockage by
biomolecular interaction that allows a sensitive electrochemical response. Here we show
the development and characterization of the nanopore-based enzymatic biosensor for the
detection of analytes. We performed and tested the concept of the simple and versatile
method of nanopores formation on the electrode using non-conductive polystyrene
nanoparticles. The variability in pore size was modulated by nanoparticles diameter (40
and 80 nm). We measured response of our designed biosensor using electrochemical
impedance spectroscopy in presence of redox mediator at low potential. The smaller
diameter of nanopores increases charge transfer resistance. Biosensors with 80 nm
nanoparticles had behaviour similar to reference without nanoparticles, therefore the pore
was too wide for this design. The limit of detection from Rct showed to be at most 0.01 mM
for every sample type. This proposed biosensor contributes to the basic knowledge about
nanopores sensors, and it could have a potential, for example, in the development of ―point
of care‖ devices.
the detection of affinity interactions such as antigen-antibody or nucleic acid hybridization.
Nanopores are frequently fabricated by anodic oxidation of aluminium. This method is
practically simple method of highly ordered pore formation, but is not suitable for massive
production. The principle of detection is usually based on the degree of pore blockage by
biomolecular interaction that allows a sensitive electrochemical response. Here we show
the development and characterization of the nanopore-based enzymatic biosensor for the
detection of analytes. We performed and tested the concept of the simple and versatile
method of nanopores formation on the electrode using non-conductive polystyrene
nanoparticles. The variability in pore size was modulated by nanoparticles diameter (40
and 80 nm). We measured response of our designed biosensor using electrochemical
impedance spectroscopy in presence of redox mediator at low potential. The smaller
diameter of nanopores increases charge transfer resistance. Biosensors with 80 nm
nanoparticles had behaviour similar to reference without nanoparticles, therefore the pore
was too wide for this design. The limit of detection from Rct showed to be at most 0.01 mM
for every sample type. This proposed biosensor contributes to the basic knowledge about
nanopores sensors, and it could have a potential, for example, in the development of ―point
of care‖ devices.
